Salivory Hormone Testing

One Day Hormone Check
What is Salivary Hormone testing?
Salivary hormone testing is an easy, non-invasive diagnostic option to measure unbound and biologically active levels of key hormones. Depending on the salivary profile selected, these include the complete array of sex hormones, with select profiles also offering adrenal hormones (cortisol and/or DHEA), and the pineal hormone, melatonin. The option to assess adrenal hormones and melatonin in addition to sex hormones provides clinical insight into how these hormones function in concert and how they may be influencing the range of presenting symptoms. Menopause Plus is Genova’s (laboratory) most comprehensive salivary hormone profile.
The One Day Hormone Check hormone test provides information about the impact that shifting hormone levels can have in men (andropause or male menopause) and women (perimenopause and menopause). In addition, imbalances of other steroid hormones, such as DHEA, cortisol, and melatonin, can cause issues across all age groups. The scientific literature confirms that hormone imbalances can be associated with common symptoms such as:
 Hot flashes
 Mood instability
 Anxiety
 Fatigue
 Cognition
 Brain fog
 Muscle weakness
 Weight gain
 Low sex drive and performance issues
 Sleep disturbances
 Altered fat metabolism
 Altered blood-sugar control
 Vaginal atrophy
 Hair Loss

 The One Day Hormone Check™ is a salivary hormone test for evaluating key hormone levels over a single day. Five specimens — collected over 24 hours — are examined for levels of estradiol, estrone, estriol, progesterone, the progesterone/estradiol ratio (P/E2), testosterone, cortisol, DHEA, and melatonin.
 To enhance clinical utility, in addition to evaluating patient results against various healthy cohort reference ranges, a therapeutic cohort results cover page is provided for the clinician to quickly and conveniently observe where patient results fall in comparison to a cohort of peri/menopausal women treated with bioidentical hormone therapy.
 Comprehensive testing: five saliva samples collected over a 24-hour period provide the level of estrone (E1), estradiol (E2), estriol (E3), progesterone, the progesterone/estradiol ratio (P/E2), testosterone, cortisol, DHEA, and melatonin
 Reporting: reference and therapeutic ranges enabling the physician to choose the optimal reference cohort for their patient
 Patient ease-of-use: saliva collection is a simple, non-invasive, at-home collection. Ideal when multiple samples are required to be collected over a period of time
What advantage does Salivary Hormone testing offer compared to other hormone tests?
Salivary assays provide several advantages over blood assays for patients with hormone-related symptoms.
 Sex hormone levels measured in saliva are thought to be unbound and bioavailable (compared to a matrix such as serum which measures both bound and unbound levels). Bioavailable hormones are hormones that can be readily absorbed/accessed by the body in support of physiological activity.
 Ease of collection makes salivary hormone sampling an ideal diagnostic for assessing diurnal variation in hormones, when multiple samples are required to be collected over a period of time.
Important things to know and consider
• Transdermal (cream) and sublingual bio-identical hormones may produce DUWLÀFLDOO\KLJKOHYHOVLQWKHVDOLYDWKDWGRQRWFRUUHODWHZLWKEORRGOHYHOV This increase from cream hormones may last for weeks to months after discontinuing use. If you are taking these substances – or have taken them within the last 12 months – please consult with your healthcare practitioner before taking this test.
The following drugs, herbs and dietary supplements may influence levels of hormones reported in this test: ketoconazole, cimetidine (Tagamet), anastrozole (Arimidex), letrozole (Femara), exemestane (Aromasin), Chrysin, Apigenin, Tribulus terrestris, clomiphene, antiepileptics, digoxin, oral steroids (e.g. Prednisone), cortisone cream, and any steroid-based nasal sprays, inhalers, or eye drops. Let your physician know about these and any other medications, herbs, and supplements that you have used in the past 3 months. Do not change use of supplements or medications unless instructed to do so by your healthcare provider.
Schedule and prepare for your saliva collection
•It is important that you collect saliva according to the Collection Schedule below. All samples must be collected within one day.
•,If you have difficulty producing saliva for the tube, press the tip of your tongue to the roof of your mouth against your teeth. Yawning can also generate saliva.
• One hour prior to each collection do not eat, brush or floss your teeth use mouthwash, chew gum or use any tobacco products. You may drink ONLY water during this time.
• Refer to the shipping instruction insert for specimen shipment instructions. Sample MUST be stored frozen at least 2 hours before shipping. •
Complete the requisition form with all patient and billing information. Be sure it is signed by the patient/responsible party and the healthcare provider.
Saliva Collection Schedule: Keep this nearby for the day!
On the day you collect specimens, wake up between 6:00 AM and 8:00
MORNING: 1 hour after rising for the day (Use label #1
MIDDAY: 11:00 AM to 1:00 PM (Use label #2)
AFTERNOON: 3:00 PM to 5:00 PM (Use label #3)
LATE NIGHT: 10:00 PM to 12:00 AM (Use label #4)
Tip: Before you go to sleep on collection day, place your collection tube (with a completed label) at your bedside, along with a glass of water and a low level light. Do not turn on a bright light, it will cause your melatonin level to drop.
EARLY NEXT MORNING: 2:30 AM to 3:30 AM (Use label #5)

SalivoryCollecting your saliva specimens
Write your full name, time, and date of collection on label #1 using a ballpoint pen or pencil only. Attach the label to a collection tube.

Test Tube Label
Test Tube Label
When ready to collect the saliva, rinse your mouth with drinking water. Spit out the water completely, and do not cough or clear your throat into the collection tube.
Mouth Cleaning
Mouth Cleaning
Fill the one collection tube until liquid saliva level reaches the 3-ml mark. To reduce a foamy saliva collection, tap the tube on a hard surface. Insufficient saliva samples cannot be processed..
Saliva Collection
Saliva Collection
Replace the cap tightly to avoid leakage. Place the filled tube into the biohazard bag and freeze immediately. Samples must be frozen a minimum of 2 hours prior to shipping. Keep sample frozen until ready to ship.
Sealing and Freezing specimen
Sealing and Freezing specimen
• Repeat steps 1-4 for each collection, using the labels numbered 2 and 3, etc., in order.
Repeat 1-4 for each collection
Repeat 1-4 for each collection
Consult your healthcare provider if you have any questions at any time during this test.
Ship the saliva specimen to the lab or bring to office if you have arranged with office.
If you are shipping then—
Specimen must be returned in the Genova Diagnostics kit box for correct delivery to the lab. Please refer to the shipping instruction insert found in your kit box.

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